AnaSpec AS-72049 AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit,AnaTag™ HiLyte™ Fluor 647蛋白標(biāo)記試劑盒-1試劑盒
AnaSpec ( 美國(guó) )
AnaSpec公司成立于1993年,是一家為*生命科學(xué)研究,提供綜合蛋白質(zhì)組學(xué)和基因組學(xué)解決方案的**供應(yīng)商。
公司產(chǎn)品主要分三大部分:提供專業(yè)多肽,檢測(cè)試劑,抗體檢測(cè)試劑盒;適合基礎(chǔ)研究的高通量篩選和新藥物研發(fā)相關(guān)試劑;多肽合成,抗體生產(chǎn),寡核苷酸的合成等定制服務(wù)。
我們北京諾博萊德科技有限公司作為AnaSpec公司的經(jīng)銷商為客戶提供,正規(guī)進(jìn)口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。
AnaSpec AS-72049 AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit,AnaTag™ HiLyte™ Fluor 647蛋白標(biāo)記試劑盒-1試劑盒,產(chǎn)品簡(jiǎn)介:
產(chǎn)品品牌:AnaSpec
產(chǎn)品名稱:AnaTag™ HiLyte™ Fluor 647蛋白標(biāo)記試劑盒-1試劑盒
英文名稱:AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit
產(chǎn)品貨號(hào):AS-72049
產(chǎn)品規(guī)格:1 kit
AnaSpec AS-72049 AnaTag™ HiLyte™ Fluor 647 Protein Labeling Kit - 1 kit,AnaTag™ HiLyte™ Fluor 647蛋白標(biāo)記試劑盒-1試劑盒,產(chǎn)品說明:
HiLyte™ Fluor 647 acid, SE (Ex/Em=647 nm/ 679 nm) is an excellent fluorescent labeling dye that generates the protein conjugates that are slightly red-shifted compared to those of Cy® 5 dyes, resulting in an optimal match to filters designed for Cy®5 dyes. However, total fluorescence of the secondary antibody conjugates of HiLyte™ Fluor 647 dyes is significantly higher than that of Cy®5 conjugates supplied by other companies. Also, unlike Cy®5 dyes, HiLyte™ Fluor 647 dyes have very little change in absorption or fluorescence spectra when conjugated to most proteins, oligonucleotides and nucleic acids, thus yielding greater total fluorescence at the same degree of substitution. The HiLyte™ Fluor 647-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte™ Fluor 647 is superior to Cy®5 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 647-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG.
Note: AnaTag kits are not intended for peptide labeling.
希利特™ 熒光素647酸,SE(Ex/Em=647 nm/679 nm)是一種優(yōu)秀的熒光標(biāo)記染料,與Cy®5染料的蛋白質(zhì)結(jié)合物相比,它產(chǎn)生的蛋白質(zhì)結(jié)合物略微紅移,從而與為Cy®5染料設(shè)計(jì)的過濾器實(shí)現(xiàn)優(yōu)良匹配。然而,HiLyte二級(jí)抗體結(jié)合物的總熒光™ Fluor 647染料明顯高于其他公司提供的Cy®5共軛物。此外,與Cy®5染料不同,HiLyte™ 當(dāng)與大多數(shù)蛋白質(zhì)、寡核苷酸和核酸結(jié)合時(shí),Fluor 647染料的吸收光譜或熒光光譜變化很小,因此在相同的取代度下產(chǎn)生更大的總熒光。希萊特™ Fluor 647蛋白偶聯(lián)物適用于免疫熒光染色、原位雜交、流式細(xì)胞術(shù)和其他生物學(xué)應(yīng)用。此外,我們的內(nèi)部數(shù)據(jù)表明,HiLyte™ Fluor 647在基于熒光偏振的分析中優(yōu)于Cy®5。該試劑盒具有用于進(jìn)行接合反應(yīng)和純化HiLyte的所有基本成分™ 氟647蛋白結(jié)合物。整個(gè)過程只需半小時(shí)。該試劑盒能夠執(zhí)行三個(gè)共軛反應(yīng),每個(gè)反應(yīng)標(biāo)記高達(dá)5 mg IgG。
注:AnaTag試劑盒不用于肽標(biāo)記。