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ScienCellGK090人體細胞連接qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):523次

GK090 ScienCell 人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK090 ScienCell人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK090

產(chǎn)品名稱:GK090 ScienCell人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK090 ScienCell人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人類細胞連接 qPCR 陣列試劑盒 (GQH-JXN) 分析了參與細胞連接形成、組織和維護的 88 個關鍵基因。 該試劑盒可研究對細胞通訊、細胞旁轉運和錨定很重要的細胞-細胞連接。 如何對所包含的基因進行分類的簡要示例如下所示:

間隙連接點: AP2M1, CLTA CLTCL1, GJA1, GJB1, PANX1

粘附連接: ANG, CADM1 CDH2, CDH5, FAT2, NECTIN2

緊密結: CDX2 CLDN1, CLDN5 JAM2 SNAI1, TJP1 ZO-1

橋粒: DSC1 DSG1, DSP, ITGB4, JUP

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance.  This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring.  Brief examples of how included genes may be categorized are shown below:

·         Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1

·         Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2

·         Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)

·         Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查詢™人類細胞連接 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

CADM1

CDH19

CELSR3

CLDN8

CXADR

DSP

GJA5

JAM3

NECTIN3

PECAM1

β-actin

B

ACTA2

CADM3

CDH2

CGN

CLTA

DCHS1

F11R

GJB1

JUP

OCLN

PVR

GAPDH

C

ACTB

CDH1

CDH3

CLDN1

CLTB

DCHS2

FAT1

GJB6

LEF1

PAK1

RHOA

LDHA

D

ACTC1

CDH10

CDH5

CLDN10

CLTC

DNM1

FAT2

ICAM1

MAGI1

PANX1

SNAI1

NONO

E

ACTG1

CDH11

CDX1

CLDN2

CLTCL1

DSC1

FAT3

ILK

MAGI2

PARD3

SP1

PPIH

F

ACTG2

CDH13

CDX2

CLDN4

CSNK1D

DSC2

FAT4

ITGB1

MYLK

PARD6A

SRC

GDC

G

ANG

CDH15

CELSR1

CLDN5

CTNNB1

DSG1

GJA1

ITGB4

NECTIN1

PCDH7

TJP1

PPC

H

AP2M1

CDH16

CELSR2

CLDN7

CTNND1

DSG3

GJA4

JAM2

NECTIN2

PCDH8

WNK4

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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GK074   人角質細胞生物學qPCR檢測試劑盒,Human Keratocyte Biology qPCR Array

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