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ScienCellGK095人間充質(zhì)至上皮細(xì)胞轉(zhuǎn)化qPCR檢測的特點
更新時間:2023-01-10   點擊次數(shù):593次

GK095 ScienCell 人間充質(zhì)至上皮細(xì)胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務(wù)標(biāo)準(zhǔn)。

GK095 ScienCell人間充質(zhì)至上皮細(xì)胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK095

產(chǎn)品名稱:GK095 ScienCell人間充質(zhì)至上皮細(xì)胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

北京裕恒豐科技有限公司是ScienCell中國區(qū)總代理,提供各種基因表達分析相關(guān)品,ScienCell細(xì)胞培養(yǎng)基、轉(zhuǎn)染試劑、細(xì)胞檢測試劑盒等細(xì)胞生物學(xué)產(chǎn)品。

GK095 ScienCell人間充質(zhì)至上皮細(xì)胞轉(zhuǎn)化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人間充質(zhì)到上皮轉(zhuǎn)化 qPCR 陣列試劑盒 (GQH-MET) 旨在促進參與可逆間充質(zhì)到上皮轉(zhuǎn)化 (MET) 的 88 個關(guān)鍵基因的基因表達譜分析。MET和反向過程,上皮到間充質(zhì)轉(zhuǎn)化(EMT),參與胚胎發(fā)生,癌癥轉(zhuǎn)移和干細(xì)胞重編程。下面顯示了如何對基因進行分組的簡要示例:    

EMT標(biāo)記: CDH1 CDH2, VIM, FN1 CDH11, SDC1 S100A4, ACTA2 CTNNB1, LEF1 ETS1, FOXC2 GSC, PDGFD

EMT-MET過渡:PAX2,WT1,BMP7TWIST1,PRRX1

增殖和形態(tài)發(fā)生控制:AKT1,BMP1,BMP7,EGFR,FGFBP1IGFBP4,JAG1,PDGFRBSNAI1,SOX10,VCAN,WNT5A,WNT11ZEB1

分化控制: BMP2, COL3A1 ERBB3, F11R, FZD7 GSC, JAG1 KRT14, 節(jié)點, SMAD2, SOX10 WNT5B

調(diào)節(jié) MET 的轉(zhuǎn)錄因子:CTNNB1、ESR1FOXC2、NOTCH1、SMAD2、SNAI1/2/3、STAT3、TCF3、TCF4、TWIST1ZEB2

MET中涉及的信號通路

雌激素受體:CAV2,ESR1,KRT19TGFB3

GPCR AKT1, FZD7, GNG11 RAC1, RGS2

PI3K/AKT AKT1 AKT2, PIK3CA PIK3CD, PIK3CG PIK3R1, PIK3R3 PIK3R5

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:    

·         EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD

·         EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1

·         Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1

·         Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B

·         Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2

·         Signaling pathways involved in MET

o    Estrogen receptor: CAV2, ESR1, KRT19, TGFB3

o    GPCR: AKT1, FZD7, GNG11, RAC1, RGS2

o    PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人間充質(zhì)到上皮過渡 qPCR 陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CAV2

COL3A1

F11R

IGFBP4

MUC1

PIK3CG

RGS2

SNAI3

TGFB2

VCAN

β-actin

B

AKT1

CDH1

COL5A2

FGFBP1

ILK

NODAL

PIK3R1

RPS27A

SOX10

TGFB3

VIM

GAPDH

C

AKT2

CDH11

CTNNB1

FN1

JAG1

NOTCH1

PIK3R3

S100A4

SPINT2

TGFBR1

WNT11

LDHA

D

ARHGEF18

CDH2

DSP

FOXC2

KRT14

PAX2

PIK3R5

SDC1

STAT3

TGFBR2

WNT5A

NONO

E

AXL

CDKN2A

EGFR

FZD7

KRT19

PDGFD

PRKCZ

SMAD2

STK11

TIMP1

WNT5B

PPIH

F

BMP1

CDKN2B

ERBB3

GNG11

LEF1

PDGFRB

PRRX1

SMURF1

TCF3

TRIM28

WT1

GDC

G

BMP2

CLDN4

ESR1

GSC

MMP2

PIK3CA

RAB25

SNAI1

TCF4

TWIST1

ZEB1

PPC

H

BMP7

CLDN7

ETS1

HNRNPAB

MST1R

PIK3CD

RAC1

SNAI2

TGFB1

UBA52

ZEB2

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

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GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

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GK020   B細(xì)胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

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GK022   人內(nèi)吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

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GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒,Human Tie2/Angpt Signaling qPCR Array Kit

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