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ScienCellGK104人類細胞骨架重塑qPCR檢測試劑盒的特點
更新時間:2023-01-10   點擊次數(shù):494次

GK104 ScienCell 人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的特約經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK104 ScienCell人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK104

產(chǎn)品名稱:GK104 ScienCell人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well

GK104 ScienCell人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit, 產(chǎn)品說明:

ScienCellGeneQuery™人類細胞骨架重塑qPCR陣列試劑盒(GQH-CYT)分析了參與細胞骨架重塑的88個關鍵基因,特別關注Rho GTP酶肌動蛋白細胞骨架重塑。 細胞骨架負責維持細胞形狀、支架和調節(jié)細胞內運輸。 它還參與許多信號通路、細胞分裂、囊泡運輸和運動。 微絲主要是肌動蛋白聚合物,是細胞骨架的一部分,由GTPRho家族調節(jié)。 如何對所包含的基因進行分類的簡要示例如下所示:

細胞骨架成分:ACTA1ACTB、ACTG1、ACTN1、MYH10、MYL1

整合素: ITGA1, ITGA3, ITGAV ITGB1, ITGB3

細胞外基質成分:COL1A1,COL2A1COL4A1,LAMA1LAMB1

Rho信號分子:CAV1,CDC42,RAC1RHOA,ROCK1,ROCK2

轉錄因子:CDX1CDX2、SP1、TCF7、TCF7L1

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO;ii)除非另有說明,否則引物集可識別靶基因的所有已知轉錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Kit (GQH-CYT) profiles 88 key genes involved in cytoskeletal remodeling with a particular focus on Rho GTPase actin cytoskeleton remodeling.  The cytoskeleton is responsible for maintaining cell shape, scaffolding, and regulating intracellular transport.  It is also involved in numerous signaling pathways, cellular division, vesicular trafficking, and motility.  Microfilaments, which are primarily actin polymers, are part of the cytoskeleton and regulated by the Rho family of GTPases.  Brief examples of how included genes may be categorized are shown below:

·         Cytoskeletal components: ACTA1, ACTB, ACTG1, ACTN1, MYH10, MYL1

·         Integrins: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3

·         Extracellular matrix components: COL1A1, COL2A1, COL4A1, LAMA1, LAMB1

·         Rho signaling molecules: CAV1, CDC42, RAC1, RHOA, ROCK1, ROCK2

·         Transcription factors: CDX1, CDX2, SP1, TCF7, TCF7L1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人類細胞骨架重塑 qPCR 陣列板布局*8 個粗體和斜體對照)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

ACTN3

CFL1

COL4A1

ITGA3

LAMB1

MYH2

MYL12B

MYLK

PTK2

SRC

β-actin

B

ACTA2

ACTN4

CFL2

COL4A2

ITGA5

LAMC1

MYH3

MYL2

MYLK2

PXN

TCF7

GAPDH

C

ACTB

ACTR3B

CLDN4

COL4A5

ITGA7

LEF1

MYH6

MYL3

MYLK3

RAC1

TCF7L1

LDHA

D

ACTC1

ARHGDIA

CLDN5

FLNA

ITGA8

LIMK1

MYH7

MYL4

MYLPF

RHOA

TCF7L2

NONO

E

ACTG1

CAV1

CLDN7

FN1

ITGAV

MAPK1

MYH7B

MYL5

NECTIN1

ROCK1

TJP1

PPIH

F

ACTG2

CDC42

COL1A1

GRB2

ITGB1

MAPK3

MYH8

MYL6

NKX2-1

ROCK2

TLN1

GDC

G

ACTN1

CDX1

COL1A2

ITGA1

ITGB3

MYH10

MYH9

MYL6B

PAK1

SNAI1

TLN2

PPC

H

ACTN2

CDX2

COL2A1

ITGA2

LAMA1

MYH11

MYL1

MYL9

PIP5K1C

SP1

VCL

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息:

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GK006   人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒,Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測試劑盒,Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉運qPCR檢測試劑盒,Human Neural Transmission and Membrane Trafficking qPCR Array Kit

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GK010   人類黑素細胞發(fā)育和色素沉著qPCR檢測試劑盒,Human Melanocyte Development & Pigmentation qPCR Array Kit

GK011   人類黑素細胞生物學qPCR檢測試劑盒,Human Melanocyte Cell Biology qPCR Array Kit

GK013   人類星型膠質細胞轉錄因子qPCR檢測試劑盒,Human Astrocyte Transcription Factors qPCR Array Kit

GK017   人血管生成qPCR檢測試劑盒,Human Angiogenesis qPCR Array Kit

GK018   人內皮細胞表型異質性qPCR檢測試劑盒,Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

GK019   人內皮細胞生物學qPCR檢測試劑盒,Human Endothelial Cell Biology qPCR Array Kit

GK020   B細胞受體信號通路qPCR檢測試劑盒,Human B Cell Receptor Signaling Pathway qPCR Array Kit

GK021   人類T細胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內吞作用qPCR檢測試劑盒,Human Endocytosis qPCR Array Kit

GK023   人皮內細胞分化qPCR檢測試劑盒,Human Endothelial Cell Differentiation qPCR Array Kit

GK024   人體剪切應力和機械傳導qPCR檢測試劑盒,Human Shear Stress and Mechanotransduction qPCR Array Kit

GK025   人類Notch信號通路qPCR檢測試劑盒,uman Notch Signaling Pathway qPCR Array Kit

GK026   人類Wnt信號通路qPCR檢測試劑盒,Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒,Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒,Human Wnt Signaling Target Genes qPCR Array Kit

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GK034   人肺纖維化qPCR檢測試劑盒,Human Pulmonary Fibrosis qPCR Array Kit

GK035   人體缺氧反應qPCR檢測試劑盒,Human Hypoxia Response qPCR Array Kit

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GK037   人巨噬細胞細胞生物學qPCR檢測試劑盒,Human Macrophage Cell Biology qPCR Array Kit

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GK040   人成纖維細胞生長因子信號通路qPCR檢測試劑盒,Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

GK041   人體皮膚傷口愈合qPCR檢測試劑盒,Human Skin Wound Healing qPCR Array Kit

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GK072   人類適應性免疫反應qPCR檢測試劑盒,Human Adaptive Immune Response qPCR Array Kit

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GK074   人角質細胞生物學qPCR檢測試劑盒,Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細胞生物學qPCR檢測試劑盒,Human Neuronal Cell Biology qPCR Array Kit

GK079   人類軟骨細胞生物學qPCR檢測試劑盒,Human Chondrocyte Biology qPCR Array Kit

GK080   人成骨分化qPCR檢測試劑盒,GeneQuery Human Osteogenic Differentiation qPCR Array Kit

GK081   人類周細胞生物學qPCR檢測試劑盒,Human Pericyte Biology qPCR Array Kit

GK084   人體軸突導向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit

GK087   人類染色質組織和重塑qPCR檢測試劑盒,Human Chromatin Organization and Remodeling qPCR Array Kit

GK088   VEGF信號通路qPCR檢測試劑盒,Human VEGF Signaling Pathway qPCR Array Kit

GK089   人類Ephrin信號qPCR檢測試劑盒,Human Ephrin Signaling qPCR Array Kit

GK090   人體細胞連接qPCR檢測試劑盒,Human Cell Junctions qPCR Array Kit

GK091   人肝星狀細胞生物學qPCR檢測試劑盒,Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array KitHuman Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR Array,Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

GK095   人間充質至上皮細胞轉化qPCR檢測試劑盒,Human Mesenchymal to Epithelial Transition qPCR Array Kit

GK096   人類雪旺細胞生物學qPCR檢測試劑盒,Human Schwann Cell Biology qPCR Array Kit

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GK125   人干細胞轉錄因子qPCR檢測試劑盒,Human Stem Cell Transcription Factors qPCR Array Kit

GK126   人類細胞表面標記qPCR檢測試劑盒,Human Cell Surface Markers qPCR Array Kit

GK127   人腦發(fā)育標記物qPCR檢測試劑盒,Human Brain Development Markers qPCR Array Kit



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