GK104 ScienCell 人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit
ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNA,cDNA和蛋白質。ScienCell的科學家們以改善生活質量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。
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GK104 ScienCell人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit,產(chǎn)品簡介:
產(chǎn)品品牌: ScienCell
產(chǎn)品貨號:GK104
產(chǎn)品名稱:GK104 ScienCell人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
GK104 ScienCell人類細胞骨架重塑qPCR檢測試劑盒,Human Cytoskeletal Remodeling qPCR Array Kit, 產(chǎn)品說明:
ScienCell的GeneQuery™人類細胞骨架重塑qPCR陣列試劑盒(GQH-CYT)分析了參與細胞骨架重塑的88個關鍵基因,特別關注Rho GTP酶肌動蛋白細胞骨架重塑。 細胞骨架負責維持細胞形狀、支架和調節(jié)細胞內運輸。 它還參與許多信號通路、細胞分裂、囊泡運輸和運動。 微絲主要是肌動蛋白聚合物,是細胞骨架的一部分,由GTP酶Rho家族調節(jié)。 如何對所包含的基因進行分類的簡要示例如下所示:
細胞骨架成分:ACTA1、ACTB、ACTG1、ACTN1、MYH10、MYL1
整合素: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3
細胞外基質成分:COL1A1,COL2A1,COL4A1,LAMA1,LAMB1
Rho信號分子:CAV1,CDC42,RAC1,RHOA,ROCK1,ROCK2
轉錄因子:CDX1、CDX2、SP1、TCF7、TCF7L1
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保:(i)qPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有說明,否則引物集可識別靶基因的所有已知轉錄本變體;(iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。
ScienCell's GeneQuery™ Human Cytoskeletal Remodeling qPCR Array Kit (GQH-CYT) profiles 88 key genes involved in cytoskeletal remodeling with a particular focus on Rho GTPase actin cytoskeleton remodeling. The cytoskeleton is responsible for maintaining cell shape, scaffolding, and regulating intracellular transport. It is also involved in numerous signaling pathways, cellular division, vesicular trafficking, and motility. Microfilaments, which are primarily actin polymers, are part of the cytoskeleton and regulated by the Rho family of GTPases. Brief examples of how included genes may be categorized are shown below:
· Cytoskeletal components: ACTA1, ACTB, ACTG1, ACTN1, MYH10, MYL1
· Integrins: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3
· Extracellular matrix components: COL1A1, COL2A1, COL4A1, LAMA1, LAMB1
· Rho signaling molecules: CAV1, CDC42, RAC1, RHOA, ROCK1, ROCK2
· Transcription factors: CDX1, CDX2, SP1, TCF7, TCF7L1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ 人類細胞骨架重塑 qPCR 陣列板布局*(8 個粗體和斜體對照)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ACTA1 | ACTN3 | CFL1 | COL4A1 | ITGA3 | LAMB1 | MYH2 | MYL12B | MYLK | PTK2 | SRC | β-actin |
B | ACTA2 | ACTN4 | CFL2 | COL4A2 | ITGA5 | LAMC1 | MYH3 | MYL2 | MYLK2 | PXN | TCF7 | GAPDH |
C | ACTB | ACTR3B | CLDN4 | COL4A5 | ITGA7 | LEF1 | MYH6 | MYL3 | MYLK3 | RAC1 | TCF7L1 | LDHA |
D | ACTC1 | ARHGDIA | CLDN5 | FLNA | ITGA8 | LIMK1 | MYH7 | MYL4 | MYLPF | RHOA | TCF7L2 | NONO |
E | ACTG1 | CAV1 | CLDN7 | FN1 | ITGAV | MAPK1 | MYH7B | MYL5 | NECTIN1 | ROCK1 | TJP1 | PPIH |
F | ACTG2 | CDC42 | COL1A1 | GRB2 | ITGB1 | MAPK3 | MYH8 | MYL6 | NKX2-1 | ROCK2 | TLN1 | GDC |
G | ACTN1 | CDX1 | COL1A2 | ITGA1 | ITGB3 | MYH10 | MYH9 | MYL6B | PAK1 | SNAI1 | TLN2 | PPC |
H | ACTN2 | CDX2 | COL2A1 | ITGA2 | LAMA1 | MYH11 | MYL1 | MYL9 | PIP5K1C | SP1 | VCL | NTC |
提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新
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